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  • Title: Two-dimensional NMR analyses of dynamic structures of tRNA and the regulation of codon recognition by post-transcriptional modifications.
    Author: Kawai G, Yokoyama S, Hasegawa T, Murao K, Ishikura H, Nishimura S, Miyazawa T.
    Journal: Nucleic Acids Symp Ser; 1988; (19):105-6. PubMed ID: 3147451.
    Abstract:
    By two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy, we analyzed dynamic structures of various tRNA species from Escherichia coli and Bacillus subtilis. Proton resonances due to the anticodon of the tRNA molecules were unambiguously identified by NOESY and 2D-HOHAHA techniques. Thus, it was found that rigidity/flexibility of the two types of modified uridines in the first position of the anticodon were certainly related with the codon recognition properties of the tRNA species.
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