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  • Title: [Separation of lymphocytes into classes from peripheral human blood using centrifugation on a discontinuous gradient].
    Author: Hadzija M, Poljak-Blazi M, Boranić M.
    Journal: Bilt Hematol Transfuz; 1978; 6(1):13-21. PubMed ID: 314801.
    Abstract:
    Peripheral blood lymphocytes of 20 normal volunteers were separated on a discontinuous gradient of Ficoll. The ability of lymphocytes from the fractions to from sponteneous (E) and induced (EAC) rosettes and undergo transformation in the presence of phytohaemagglutinin (PHA) was tested. T-lymphocytes were concentrated in the density of 1,067 g/ml and B-lymphocytes in the density of 1,063 g/ml.
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