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  • Title: A cortical granule-specific enzyme, B-1,3-glucanase, in sea urchin eggs.
    Author: Wessel GM, Truschel MR, Chambers SA, McClay DR.
    Journal: Gamete Res; 1987 Dec; 18(4):339-48. PubMed ID: 3148543.
    Abstract:
    The ultrastructural localization of B-1,3-glucanase in three species of sea urchin eggs was determined using a monospecific antibody in an electronmicroscopic immunogold procedure. In all three species, Lytechinus variegatus, Strongylocentrotus purpuratus, and Arbacia punctulata, B-1,3-glucanase was localized specifically to the cortical granules. No other organelle within the egg contained significant label. During the fertilization reaction, B-1,3-glucanase was released from cortical granules into the perivitelline space and became associated with the hyaline layer. No significant label was found in association with the fertilization envelope.
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