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  • Title: Direct electrochemistry of glucose oxidase based on one step electrodeposition of reduced graphene oxide incorporating polymerized l-lysine and its application in glucose sensing.
    Author: Zhang D, Chen X, Ma W, Yang T, Li D, Dai B, Zhang Y.
    Journal: Mater Sci Eng C Mater Biol Appl; 2019 Nov; 104():109880. PubMed ID: 31500020.
    Abstract:
    The direct electron transfer and enzyme catalytic activity were investigated in electrochemical biosensor based on glucose oxidase (GOx) and electrochemical reduced graphene oxide-poly-(l-lysine) (ERGO-PLL) hybrid composite film embedded in a biocompatible matrix of Nafion. The ERGO-PLL was fabricated onto glassy carbon electrode (GCE) through one step electrodeposition of RGO incorporating PLL from graphene oxide-l-lysine aqueous dispersion. The fabrication process of Nafion/GOx/ERGO-PLL/GCE has been characterized by cyclic voltammetry and electrochemical impedance spectroscopy, and the surface morphology of modified electrodes were characterized by scanning electron microscopy. A pair of well-defined redox peaks with formal potential and peak separation of -0.461 V and 30 mV were observed at scan rate of 50 mV s-1, the electron transfer rate constant was calculated to be 18.7 s-1, demonstrated that direct electron transfer between immobilized GOx with ERGO-PLL and GCE was achieved. Moreover, the fabricated enzyme biosensor exhibited excellent electrocatalytic activity for determination of glucose in O2-free PBS (7.40) with linear voltammetric response from 0.005 to 9.0 mmol L-1, and detection limits (S/N = 3) of 2.0 μmol L-1. This work indicates that ERGO-PLL based modified electrode is superior to that of graphene based, and could be applied in biosensors, bioelectronics and electrocatalysis, etc.
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