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  • Title: The chemiluminescent response of human monocytes to red cells sensitized with monoclonal anti-Rh(D) antibodies.
    Author: Hadley AG, Kumpel BM, Merry AH.
    Journal: Clin Lab Haematol; 1988; 10(4):377-84. PubMed ID: 3150696.
    Abstract:
    Luminol-enhanced chemiluminescence (CL) was used to assess the metabolic response of human monocytes to red cells sensitized with known amounts of anti-Rh(D). Monoclonal antibodies were used to facilitate a comparison between the functional activities of IgG1 and IgG3 subclasses. The detection of CL provided a simple, rapid and semi-quantitative means of measuring monocyte response to sensitized red cells (IgG-RBC). Monocyte response to IgG3-RBC was quantitatively greater, more rapid and less susceptible to inhibition by fluid phase IgG than monocyte response to IgG1-RBC. The minimum levels of sensitization required to elicit CL from monocytes were approximately 2500 IgG3 molecules per red cell, or approximately 5000 IgG1 molecules per cell.
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