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  • Title: Mitochondria-Anchored Colorimetric and Ratiometric Fluorescent Chemosensor for Visualizing Cysteine/Homocysteine in Living Cells and Daphnia magna Model.
    Author: Yang M, Fan J, Sun W, Du J, Peng X.
    Journal: Anal Chem; 2019 Oct 01; 91(19):12531-12537. PubMed ID: 31507158.
    Abstract:
    Cysteine (Cys) and homocysteine (Hcy) are essential for maintaining the cellular redox homeostasis and play critical roles in pathological and physiological processes. The development of Cys/Hcy-specific responsive fluorescent probes that are independent of the surrounding environment, equipment, and abundant endogenous GSH is critical to accurately investigate the roles of Cys/Hcy in living biological systems. In this work, a novel ratiometric and mitochondria-anchored fluorescence chemosensor, PYR, was constructed on the basis of 4-methylphenol-substituted pyronin fluorophore. The probe exhibited ratiometric fluorescence emission (F540 nm/F620 nm) for the detection of Cys/Hcy with high selectivity, sensitivity (Cys, 22 nM; Hcy, 23 nM), rapid response (Cys, 5 min), and a merit enhancement of ratio fluorescent signal (Cys, 163-fold; Hcy, 125-fold). The probe showed excellent membrane permeability and was applied to visualize mitochondrial biothiols in living cells under H2O2-induced redox imbalance, kidney tissues with a penetration depth of 100 μm, and Daphnia magna model for the first time. The results demonstrate that PYR will provide a promising platform for the diagnosis of thiol-related diseases.
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