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  • Title: Quantification of ovine herpesvirus 2 by digital PCR in an outbreak of malignant catarrhal fever.
    Author: Pinheiro de Oliveira TF, Laguardia-Nascimento M, Xavier FG, do Amaral Pinto C, Ferreira LR, de Castro Campos de Souza I, Hammerschmitt ME, Bianchi RM, Wronski JG, Etges RN, Rigon GM, Camargos MF, Júnior AVR, Fonseca Junior AA.
    Journal: Arch Virol; 2019 Dec; 164(12):3045-3050. PubMed ID: 31520217.
    Abstract:
    Infection with ovine gammaherpesvirus 2 (OvHV-2) is generally asymptomatic in sheep; however, when it crosses the species barrier, it causes malignant catarrhal fever (MCF) in cattle. In the present study, we developed a real-time PCR assay and a droplet digital PCR assay and use both methods to study an outbreak caused by OvHV-2. Both PCR methods showed high sensitivity and specificity and were able to detect low copy numbers of OvHV-2 in sheep and cattle. The present study describes the first digital PCR quantification of OvHV-2 genome copies in samples collected from sheep and cattle.
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