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  • Title: Direct observation of the NAD glycohydrolase reaction in human erythrocytes using NMR spectroscopy.
    Author: Kuchel PW, Chapman BE.
    Journal: Experientia; 1985 Jan 15; 41(1):53-5. PubMed ID: 3155692.
    Abstract:
    The hydrolysis of NAD by the extracellular membrane-associated enzyme NAD glycohydrolase was shown to be readily followed in concentrated suspensions of human erythrocytes using 1H spin-echo nuclear magnetic resonance spectroscopy (NMR). The maximal rate of the reaction was determined and the inhibitory effect of nicotinamide was confirmed by direct NMR observation. In addition, arginine, ergothioneine and iodoacetate did not influence the reaction rate. 31P NMR analyses of reaction media from whole cells showed that no extraneous degradation of NAD occurred and the only phosphate-containing product was ADP-ribose.
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