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  • Title: Immunoassay of rod visual pigment (opsin) in the eyes of rds mutant mice lacking receptor outer segments.
    Author: Schalken JJ, Janssen JJ, de Grip WJ, Hawkins RK, Sanyal S.
    Journal: Biochim Biophys Acta; 1985 Mar 29; 839(1):122-6. PubMed ID: 3156637.
    Abstract:
    In 020/A mice, homozygous for the retinal degeneration slow (rds) gene, the photoreceptor cells fail to develop outer segments, and in the absorption spectra of retinal extracts the rhodopsin peak is lacking. Application of an enzyme-linked immunoassay using antisera against bovine opsin shows, however, that opsin is present in the homozygous mutant retina (0.010 nmol/eye) at 3% of the level of the normal retina (0.38 nmol/eye) of Balb/c mice. In the retina of heterozygous mice the opsin level (0.19 nmol/eye) is about half of the normal. Detection of opsin in the rds mutant retina demonstrates the functional basis for the reported electroretinographic response and light-mediated reduction in cyclic nucleotide levels in this mutant.
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