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  • Title: Super-Resolution Visualization of Self-Assembling Helical Fibers Using Aggregation-Induced Emission Luminogens in Stimulated Emission Depletion Nanoscopy.
    Author: Dang D, Zhang H, Xu Y, Xu R, Wang Z, Kwok RTK, Lam JWY, Zhang L, Meng L, Tang BZ.
    Journal: ACS Nano; 2019 Oct 22; 13(10):11863-11873. PubMed ID: 31584798.
    Abstract:
    Organic fluorophores for stimulated emission depletion (STED) nanoscopy usually suffer from quenched emission in the aggregate state and inferior photostability, which largely limit their application in real-time, in situ, and long-term imaging at an ultrahigh resolution. Herein, an aggregation-induced emission (AIE) luminogen of DP-TBT with bright emission in solid state (photoluminescence quantum yields = 25%) and excellent photostability was designed to meet the requirements in STED nanoscopy. In addition to its excellent fluorescence properties, DP-TBT could also easily form self-assembling helixes and finally be well-visualized by super-resolution STED nanoscopy. The observations showed that helical fibers of DP-TBT as dashed lines had a much decreased fiber width with also a full width at half-maximum value of only 178 nm, which is ∼6 times higher than solid lines obtained by confocal microscopy (1154 nm). The STED nanoscopic data were also used to reconstruct 3D images of assembled helixes. Finally, by long-term tracking and dynamic monitoring, the formation and growth of helical fibers by DP-TBT in self-assembly processes were successfully obtained. These findings imply that highly emissive AIEgens with good photostability are highly suitable for real-time, in situ, and dynamic imaging at super-resolution using STED nanoscopy.
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