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  • Title: Isolation of two forms of basement membrane proteoglycans.
    Author: Hassell JR, Leyshon WC, Ledbetter SR, Tyree B, Suzuki S, Kato M, Kimata K, Kleinman HK.
    Journal: J Biol Chem; 1985 Jul 05; 260(13):8098-105. PubMed ID: 3159724.
    Abstract:
    Sequential extractions of the basement membrane producing Engelbreth-Holm-Swarm tumor yielded heparan sulfate proteoglycans with different size core proteins, but the same size heparan sulfate side chains. Saline, a nondenaturing solvent, extracted a small high density proteoglycan with a heterodisperse core protein of Mr = 95,000-130,000 whereas subsequent extraction with 7 M urea, a denaturing solvent, removed a large, low density proteoglycan with a Mr = 350,000-400,000 protein core. The denaturing conditions required for extraction of the large proteoglycan suggest that it interacts strongly with other basement membrane components. Antibodies to these proteoglycans cross-react with both proteoglycans, but the large proteoglycan has additional antigenic sites not present on the small proteoglycan. These proteoglycans may be derived from the same or similar gene products.
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