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  • Title: Metabolomic Analysis of Yeast and Human Cells: Latest Advances and Challenges.
    Author: Chaleckis R, Ohashi K, Meister I, Naz S, Wheelock CE.
    Journal: Methods Mol Biol; 2019; 2049():233-245. PubMed ID: 31602615.
    Abstract:
    Liquid chromatography-mass spectrometry (LC-MS) based nontargeted metabolomics has been applied to a wide range of biological samples and can provide information on thousands of compounds. However, reliable identification of the compounds remains a challenge affecting result interpretation. In this protocol, we describe comparable yeast cell and whole blood metabolome sample preparation for extracting similar compound groups, and we present a LC-MS method using the all ion fragmentation (AIF) approach for the purposes of increasing accuracy in metabolite annotation. Our method enables database-dependent targeted as well as nontargeted metabolomics analysis from the same data acquisition, while simultaneously improving the accuracy in metabolite identification to increase the quality of the resulting biological information.
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