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  • Title: Cloning and expression of two Streptococcus mutans glucosyltransferases in Escherichia coli K-12.
    Author: Gilpin ML, Russell RR, Morrissey P.
    Journal: Infect Immun; 1985 Aug; 49(2):414-6. PubMed ID: 3160662.
    Abstract:
    Chromosomal DNA from Streptococcus mutans strain MFe28 (serotype h) was cloned in the bacteriophage vector lambda L47.1. Two classes of recombinants were found which expressed glucosyltransferase activity in phage plaques: (i) gtfS, which expressed a glucosyltransferase synthesizing a water-soluble, dextranase-sensitive glucan, and (ii) gtfI, which expressed a primer-dependent glucosyltransferase synthesizing an insoluble glucan.
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