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  • Title: mEosEM withstands osmium staining and Epon embedding for super-resolution CLEM.
    Author: Fu Z, Peng D, Zhang M, Xue F, Zhang R, He W, Xu T, Xu P.
    Journal: Nat Methods; 2020 Jan; 17(1):55-58. PubMed ID: 31611693.
    Abstract:
    Super-resolution correlative light and electron microscopy (SR-CLEM) is a powerful approach for imaging specific molecules at the nanoscale in the context of the cellular ultrastructure. Epon epoxy resin embedding offers advantages for SR-CLEM, including ultrastructural preservation and high quality sectioning. However, Epon embedding eliminates fluorescence from most fluorescent proteins. We describe a photocontrollable fluorescent protein, mEosEM, that can survive Epon embedding after osmium tetroxide (OsO4) treatment for improved SR-CLEM.
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