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  • Title: [Inhibition of Toxoplasma gondii excretory - secretory antigens on growth of murine Lewis lung carcinoma].
    Author: Yu-Meng J, Zhi-Yong T, Yu-Jian C, Chun-Xiang L, Hui X, Xue-Mei W, Qiang F.
    Journal: Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi; 2019 Sep 23; 31(4):400-403. PubMed ID: 31612675.
    Abstract:
    OBJECTIVE: To investigate the effect of Toxoplasma gondii excretory-secretory antigens (ESA) on CD4+ CD25+ Foxp3+ T (Treg) cells in mice carrying Lewis lung carcinoma, and examine the inhibitory effect of T. gondii ESA on tumor growth. METHODS: C57BL/6 mice were randomly assigned into the PBS group (n = 14) and the Lewis group (n = 34). Mice in the Lewis group were subcutaneously injected with 2 × 105 Lewis lung carcinoma cells in the right axilla, while animals in the PBS group were injected with the same volume of sterile PBS. On day 7 post-injection (D7), mice in the PBS group were further divided into the PBS2 group and the PBS2 + ESA group, of 7 mice in each group, and mice in the Lewis group were further divided into the Lewis2 group and the Lewis2 + ESA group, of 17 mice in each group. Then, mice in the PBS2 + ESA group and the Lewis2 + ESA group were intraperitoneally injected with 100 μL of ESA. The mouse spleen coefficient was calculated in each group 7 days post-injection with ESA, and the changes of Treg cell counts and the long-term tumor growth were measured in tumor-bearing mice. RESULTS: The spleen coefficient was significantly greater in the PBS2 + ESA group and the Lewis2 + ESA group than in the PBS2 (0.66% ± 0.09% vs. 0.30% ± 0.02%, P < 0.05) and Lewis2 groups (0.69% ± 0.07% vs. 0.33% ± 0.03%, P < 0.05) 7 days post-treatment with ESA, respectively, and the percentage of splenic Treg cells in splenocytes was significantly lower in the PBS2 + ESA group and the Lewis2 + ESA group than in the PBS2 (1.28% ± 0.14% vs. 2.06% ± 0.07%, P < 0.05) and Lewis2 groups (1.58% ± 0.14% vs. 2.44% ± 0.23%, P < 0.05), respectively. T. gondii ESA treatment caused a delay in tumor growth, and the tumor size was significantly smaller in the Lewis2 + ESA group than in the Lewis2 group (P < 0.05). CONCLUSIONS: T. gondii ESA may reduce the proportion of splenic Treg cells in splenocytes and inhibit tumor growth in mice carrying Lewis lung carcinoma. [摘要] 目的 探讨弓形虫排泄分泌抗原 (ESA) 对 Lewis肺癌小鼠CD4+ CD25+ Foxp3+ T (Treg) 细胞亚群的影响, 观察弓 形虫ESA对肿瘤生长的抑制作用。方法 将C57BL/6小鼠随机分成PBS组 (14只) 和Lewis组 (34只)。Lewis组小鼠右腋 窝皮下接种Lewis肺癌细胞2 × 105个, PBS组注射等量无菌PBS。接种后第7 天 (D7), 将PBS组小鼠分成PBS2组、PBS2 + ESA组, 每组7只; 将Lewis组分成Lewis2组和Lewis2 + ESA组, 每组17只; PBS2 + ESA组及Lewis2 + ESA组小鼠腹腔注 射100 μL弓形虫ESA。ESA干预后7 d计算各组小鼠脾脏系数, 检测Treg细胞数量变化, 同时观察荷瘤鼠长期瘤体生长 情况。结果 ESA干预后7 d, PBS2 + ESA组 [(0.66 ± 0.09) %]和 Lewis2 + ESA组 [(0.69 ± 0.07) %]脾脏明显增大, 脾脏系 数与PBS2 组 [(0.30 ± 0.02) %]和Lewis2 组 [(0.33 ± 0.03) %]比较, 差异均有统计学意义 (P 均< 0.05)。PBS2 + ESA 组 [(1.28 ± 0.14) %]和Lewis2 + ESA组 [(1.58 ± 0.14) %]脾脏Treg细胞占脾细胞的比例均下降, 与PBS2组 [(2.06 ± 0.07) %]和 Lewis2组 [(2.44 ± 0.23) %]比较差异均有统计学意义 (P 均< 0.05)。ESA干预后, 瘤体生长延缓, 在实验终点时, Lewis2 + ESA组小鼠瘤体明显小于Lewis2组 (P < 0.05)。结论 ESA可下调荷瘤鼠脾脏Treg细胞占脾细胞的比例, 抑制瘤体生 长。.
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