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  • Title: Production of rosmarinic acid with ATP and CoA double regenerating system.
    Author: Yan Y, Jia P, Bai Y, Fan TP, Zheng X, Cai Y.
    Journal: Enzyme Microb Technol; 2019 Dec; 131():109392. PubMed ID: 31615678.
    Abstract:
    Rosmarinic acid (RA), as a hydroxycinnamic acid ester of caffeic acid (CA) and 3,4-dihydroxyphenyllactic acid (3,4-DHPL), is a phenylpropanoid-derived plant natural product and has diverse biological activities. This work acts as a modular platform for microbial production using a two-cofactor (ATP and CoA) regeneration system to product RA based on a cell-free biosynthetic approach. Optimal activity of the reaction system was pH 8 and 30 °C. Total turnover number for ATP and CoA was 820.60 ± 28.60 and 444.50 ± 9.65, respectively. Based on the first hour data, the RA productivity reached 320.04 mg L-1 h-1 (0.889 mM L-1 h-1).
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