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  • Title: Rapid and sensitive determination of zearalenone in cereals by high-performance liquid chromatography with fluorescence detection.
    Author: Tanaka T, Hasegawa A, Matsuki Y, Lee US, Ueno Y.
    Journal: J Chromatogr; 1985 Jun 28; 328():271-8. PubMed ID: 3161904.
    Abstract:
    Zearalenone, an estrogenic mycotoxin of Fusarium species, in cereals can be extracted with acetonitrile-water (3:1), purified on a Florisil column, resolved by high-performance liquid chromatography (HPLC) with a Nucleosil 50-10 column using 90% water-saturated chloroform-cyclohexane-acetonitrile-ethanol (50:15:2:1) and quantitated by fluorescence measurement. This method is rapid, simple and reproducible, and detects zearalenone in wheat, barley, corn and other cereals with picogram sensitivity. A combination of this HPLC method with a gas-liquid chromatographic method for trichothecenes may be applied to the simultaneous detection of Fusarium mycotoxins (zearalenone, nivalenol and deoxynivalenol) in cereals.
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