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  • Title: Electrochemical biosensor for amplified detection of Pb2+ based on perfect match of reduced graphene oxide-gold nanoparticles and single-stranded DNAzyme.
    Author: Lai C, Zhang Y, Liu X, Liu S, Li B, Zhang M, Qin L, Yi H, Li M, Li L, Fu Y, He J, Chen L.
    Journal: Anal Bioanal Chem; 2019 Nov; 411(28):7499-7509. PubMed ID: 31637461.
    Abstract:
    In this study, a sensitive amplification strategy for Pb2+ detection using reduced graphene oxide (RGO) and gold nanoparticles (AuNPs) was proposed. Thiol-modified DNAzyme is specific for Pb2+ self-assembly on RGO-AuNPs-modified electrode surface. Ferrocene labeled single-stranded DNAzyme (Fc-ssDNAzyme) self-hybridizes to form a DNA hairpin structure. The amount of Fc adsorbed on the electrode surface changes after the appearance of Pb2+, leading to a change of electrical signal. This change can be sensitively identified by differential pulse voltammetry (DPV) assisted by ferricyanide ([Fe(CN)6]3-/4-) in the electrolyte. The high conductivity and specific surface area of RGO and the strong chemical bond adsorption effect between DNAzyme and AuNPs are responsible for the amplified detection of Pb2+, which realize a detection range of 0.05-400,000.0 nM and a minimum detection limit of 0.015 nM. Moreover, the selectivity test results indicated that the biosensor had specificity for Pb2+, even if there was interference from other high-concentration metal ions. This simple biosensor also exhibited good responsiveness in actual sample detection, which provides a good application prospect for field detection of Pb2+ in water. Graphical abstract.
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