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  • Title: Determination of 4,4'-methylenebis(2-chloroaniline) in urine by liquid chromatography with ion-paired solid-phase extraction and electrochemical detection.
    Author: Okayama A, Ichikawa Y, Yoshida M, Hara I, Morimoto K.
    Journal: Clin Chem; 1988 Oct; 34(10):2122-5. PubMed ID: 3168228.
    Abstract:
    This highly specific and sensitive method for measuring urinary 4,4'-methylenebis(2-chloroaniline) (MBOCA) involves liquid chromatography with electrochemical detection. Before chromatography, urine samples are prepared by ion-paired solid-phase extraction on a disposable octadecylsilica column with acidic methanol solution containing 1-heptanesulfonic acid. This enhances the specificity of the method. Mean overall recovery ranged from 97.1% to 99.5% at added MBOCA concentrations of 20 and 100 micrograms/L in urine. Sensitivity for urine was 1 microgram/L. The intra-assay CV was 2.2% at a MBOCA concentration of 100 micrograms/L. We believe that this method is acceptable for routine measurement of MBOCA in urine from individuals exposed to this industrial chemical.
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