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  • Title: Method for growing primitive erythroid progenitors (BFU-E) from rat bone marrow.
    Author: Basara N, Biljanović-Paunović L, Pavlović-Kentera V.
    Journal: Exp Hematol; 1988 Oct; 16(9):790-3. PubMed ID: 3169160.
    Abstract:
    A method for growing erythroid bursts from rat bone marrow cells is presented. The methylcellulose culture technique with rat spleen-conditioned medium was used. Successful growth of rat BFU-E was obtained with 20% fetal bovine serum when anemic rat plasma was used as the source of erythropoietin. Plating efficiency was 60 BFU-E per 2 x 10(5) nucleated cells seeded. The proliferative activity determined by cytosine arabinoside (Ara-C) suicide was about 22%. The effect of chronic hypertransfusion on erythroid colony formation is presented.
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