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  • Title: A convenient in vivo cap donor delivery system to investigate the cap snatching of plant bunyaviruses.
    Author: Lin W, Wu R, Qiu P, Jing Jin, Yang Y, Wang J, Lin Z, Zhang J, Wu Z, Du Z.
    Journal: Virology; 2020 Jan 02; 539():114-120. PubMed ID: 31710910.
    Abstract:
    Like their animal-infecting counterparts, plant bunyaviruses use capped RNA leaders cleaved from host cellular mRNAs to prime viral genome transcription in a process called cap-snatching, but in vivo systems to investigate the details of this process are lacking for them. Here, we report that Rice stripe tenuivirus (RSV) and Tomato spotted wilt tospovirus (TSWV) cleave capped RNA leaders from mRNAs transiently expressed by agroinfiltration, which makes it possible to artificially deliver defined cap donors to the two plant bunyaviruses with unprecedented convenience. With this system, some ideas regarding how plant bunyaviruses select and use capped RNA leaders can be tested easily. We were also able to obtain clear evidence that the capped RNA leaders selected by TSWV are generally longer than those by RSV. TSWV frequently uses the prime-and-realign mechanism in transcription primed by capped RNA leaders shorter than a certain length, like that has been demonstrated recently for RSV.
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