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  • Title: In vitro blastogenesis of porcine peripheral blood lymphocytes.
    Author: Onyekaba C.
    Journal: Microbiologica; 1988 Jul; 11(3):201-5. PubMed ID: 3173123.
    Abstract:
    A microculture technique was used for the in vitro blastogenesis of pig lymphocytes. The effects of some variable on the stimulation of L luminal diameter culture were compared. A cell concentration of 4 x 10(6) cells/well gave an optimal stimulation index (S.I.) of 121.3, with 50 micrograms/ml of concanavalin A (Con A), whereas cell concentration of 5 x 10(5) and concanavalin concentration of 100 micrograms/ml gave the lowest stimulation of 1.61. Good stimulation was observed in almost all cultures incubated for 48 hrs and labelled with 1 Mci triated thymidine (3HTdr) for the last 8 hrs. The highest S.I. were in the range of 66.3 to 121.3 (73,223 to 133,893 counts per minute) for the pig lymphocytes derived from whole blood culture.
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