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  • Title: RNA mediated formation of a phosphorothioate diester bond.
    Author: Buzayan JM, Feldstein PA, Bruening G, Eckstein F.
    Journal: Biochem Biophys Res Commun; 1988 Oct 14; 156(1):340-7. PubMed ID: 3178838.
    Abstract:
    Previous results showed that multimeric, tandemly sequence-repeated forms of satellite tobacco ringspot virus RNA of the encapsidated polarity (STobRV (+)RNA) autolytically process at a specific phosphodiester bond, the junction. Substituting a phosphorothioate diester bond for the STobRV (+)RNA junction drastically slowed autolytic processing. Here we show that for the complementary STobRV (-)RNA, in contrast, replacing sets of phosphodiester bonds with phosphorothioate diester bonds, even at the junction, did not greatly slow autolytic processing or spontaneous ligation, the usual reactions of the unmodified RNA. In the ligation reaction STobRV (-)RNA directed the formation of an ApG phosphorothioate diester bond.
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