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  • Title: Evaluation of the Unyvero i60 ITI® multiplex PCR for infected chronic leg ulcers diagnosis.
    Author: Reissier S, Lazareth I, Adjiman L, Couzigou C, Vidal B, Mizrahi A, Nguyen Van JC, Pilmis B, Le Monnier A, Lourtet-Hascoët J.
    Journal: J Microbiol Methods; 2020 Jan; 168():105796. PubMed ID: 31790779.
    Abstract:
    OBJECTIVES: Unyvero i60 ITI multiplex PCR (mPCR) may identify a large panel of bacteria and antibiotic resistance genes. In this study, we compared results obtained by mPCR to standard bacteriology in chronic leg ulcer (CLU) infections. METHODS: A prospective study, part of the interventional-blinded randomized study "ulcerinfecte" (NCT02889926), was conducted at Saint Joseph Hospital in Paris. Fifty patients with a suspicion of infected CLU were included between February 2017 and September 2018. Conventional bacteriology and mPCR were performed simultaneously on deep skin biopsies. RESULTS: Staphylococcus aureus and Pseudomonas aeruginosa were the most detected pathogens. Regarding the global sensitivity, mPCR is not overcome to the standard culture. Anaerobes and slow growing bacteria were detected with a higher sensitivity rate by mPCR than standard culture. CONCLUSION: Unyvero i60 ITI multiplex PCR detected rapidly pathogenic bacteria in infected CLU especially anaerobes and slow growing bacteria and was particularly effective for patients previously treated with antibiotics.
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