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  • Title: Next-generation Interactomics: Considerations for the Use of Co-elution to Measure Protein Interaction Networks.
    Author: Salas D, Stacey RG, Akinlaja M, Foster LJ.
    Journal: Mol Cell Proteomics; 2020 Jan; 19(1):1-10. PubMed ID: 31792070.
    Abstract:
    Understanding how proteins interact is crucial to understanding cellular processes. Among the available interactome mapping methods, co-elution stands out as a method that is simultaneous in nature and capable of identifying interactions between all the proteins detected in a sample. The general workflow in co-elution methods involves the mild extraction of protein complexes and their separation into several fractions, across which proteins bound together in the same complex will show similar co-elution profiles when analyzed appropriately. In this review we discuss the different separation, quantification and bioinformatic strategies used in co-elution studies, and the important considerations in designing these studies. The benefits of co-elution versus other methods makes it a valuable starting point when asking questions that involve the perturbation of the interactome.
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