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  • Title: A molecular mechanism for the procentriole recruitment of Ana2.
    Author: McLamarrah TA, Speed SK, Ryniawec JM, Buster DW, Fagerstrom CJ, Galletta BJ, Rusan NM, Rogers GC.
    Journal: J Cell Biol; 2020 Feb 03; 219(2):. PubMed ID: 31841145.
    Abstract:
    During centriole duplication, a preprocentriole forms at a single site on the mother centriole through a process that includes the hierarchical recruitment of a conserved set of proteins, including the Polo-like kinase 4 (Plk4), Ana2/STIL, and the cartwheel protein Sas6. Ana2/STIL is critical for procentriole assembly, and its recruitment is controlled by the kinase activity of Plk4, but how this works remains poorly understood. A structural motif called the G-box in the centriole outer wall protein Sas4 interacts with a short region in the N terminus of Ana2/STIL. Here, we show that binding of Ana2 to the Sas4 G-box enables hyperphosphorylation of the Ana2 N terminus by Plk4. Hyperphosphorylation increases the affinity of the Ana2-G-box interaction, and, consequently, promotes the accumulation of Ana2 at the procentriole to induce daughter centriole formation.
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