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  • Title: Cloning and sequence analysis of cDNA for corticotropin-releasing factor precursor from the teleost fish Catostomus commersoni.
    Author: Okawara Y, Morley SD, Burzio LO, Zwiers H, Lederis K, Richter D.
    Journal: Proc Natl Acad Sci U S A; 1988 Nov; 85(22):8439-43. PubMed ID: 3186733.
    Abstract:
    The sequence of a cDNA encoding the corticotropin-releasing factor precursor has been identified by screening lambda gt11 libraries constructed from poly(A)+ RNA of the hypothalamic region of the white sucker Catostomus commersoni brain with synthetic oligonucleotide probes deduced from the sequence of the rat corticotropin-releasing factor. The amino acid sequence of corticotropin-releasing factor of the sucker is strikingly conserved when compared to its counterpart from rat and differs only in two positions at the carboxyl terminus; in contrast, there is little similarity between their cryptic regions.
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