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  • Title: beta-Bungarotoxin-induced phospholipid hydrolysis in rat brain synaptosomes: effect of replacement of calcium by strontium.
    Author: Ghassemi A, Dhillon DS, Rosenberg P.
    Journal: Toxicon; 1988; 26(5):509-14. PubMed ID: 3188058.
    Abstract:
    We tested whether, upon substitution of Ca2+ by Sr2+ in a medium containing beta-bungarotoxin, sufficient Ca2+ remained bound to the tissue to support phospholipid hydrolysis in rat brain synaptosomes. The phrenic nerve--diaphragm preparation could not be used, since replacement of Ca2+ by Sr2+ prolonged time to block of indirectly evoked contractions; however, no phospholipid hydrolysis could be detected (either in the presence of Ca2+ or Sr2+), due to the small amounts of presynaptic terminals. Following initial exposure of synaptosomes to a Ca2+ containing medium and then removal of Ca2+, incubation with beta-bungarotoxin (1 or 10 micrograms/ml) caused significant phospholipid hydrolysis whether or not Sr2+ was present. Therefore, conclusions as to whether phospholipase A2 activity is required for presynaptic actions of beta-bungarotoxin cannot be derived from studies in which Sr2+ is used to inhibit enzymatic activity.
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