These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: An electrochemical aptasensor for analysis of MUC1 using gold platinum bimetallic nanoparticles deposited carboxylated graphene oxide.
    Author: Bharti A, Rana S, Dahiya D, Agnihotri N, Prabhakar N.
    Journal: Anal Chim Acta; 2020 Feb 08; 1097():186-195. PubMed ID: 31910959.
    Abstract:
    A simple electrochemical strategy has been designed for the analysis of MUC1 using electrodeposited gold platinum bimetallic nanoparticles (Au-PtBNPs) on the surface of carboxylated graphene oxide (CGO)/FTO electrode as a signal amplification platform. The carboxylic groups of CGO were activated with EDS-NHS linker and subsequently immobilized with streptavidin for further deposition of biotin labelled aptamer. All the modification steps were characterized by FE-SEM, EDS mapping, FT-IR, contact angle measurements and electrochemical methods. After incubating with target protein MUC1, the aptaelectrode produced some concentration dependent responses which were measured electrochemically by DPV assay. The prepared aptasensor exhibits wide linear range from 1 fM-100 nM with detection limit of 0.79 fM under optimal experimental conditions. The performance of this aptaelectrode was also evaluated showing good selectivity, storage stability (15 days), reproducibility and reusability (up to 3 times). Furthermore, the applicability of the aptasensor for spiked serum samples showed recovery range from 92% to 97%.
    [Abstract] [Full Text] [Related] [New Search]