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Title: [Effect of TUTF1 expression on the proliferation, apoptosis and prognosis of hepatocellular carcinoma cells].
Author: Li ZJ, Li JS, Li DY, Pu T.
Journal: Zhonghua Gan Zang Bing Za Zhi; 2019 Nov 20; 27(11):879-884. PubMed ID: 31941243.
Abstract:
Objective: To study the relationship between the expressions of tuftelin 1 (TUFT1) and the clinicopathological features of hepatocellular carcinoma and its effect on proliferation and apoptosis, and to explore the relationship between TUFT1 with the development of hepatocellular carcinoma. Methods: Immunohistochemistry was used to detect the expression of TUFT1 in 98 cases of hepatocellular carcinoma and 30 cases of adjacent normal tissues. Quantitative real-time PCR (qRT-PCR) was used to detect the expression of TUFT1 in HCC cell line. The expression of TUFT1 in SMMC-7221 cell lines was down-regulated by lentiviral vector. Cell proliferation assay, clonogenic assay, cell apoptosis assay and cell cycle assay were used to detect proliferation, apoptosis, and cell cycle changes of hepatocarcinoma cells after TUFT1-down-regulation. Statistics were performed using the χ2 test and the t-test. Results: Among the 98 cases of hepatocellular carcinoma, 65 cases (66.33%) were positive for TUFT1, and in 30 cases of adjacent normal tissues, 6 cases (16.67%) were positive for TUFT1, and the difference was statistically significant (χ (2) = 19.956, P < 0.05). The expression of TUFT1 in HCC tissues was related to tumor size, tumor stage, recurrence and metastasis (χ(2) = 6.214, 8.066, 14.400, P < 0.05). After lentiviral vector mediated downregulation of TUFT1 expression in SMMC -7221 cells, the cell proliferation rate [(18.62% ± 0.15%) vs. (67.91% ± 0.62%), P < 0.05], clonality [(8.10% ± 0.80%) vs. (50.80% ± 1.60%), P < 0.05] and G1 phase cells [(36.71% ± 0.69%) vs. (44.65% ± 0.73%), P < 0.05] were significantly decreased, whereas the G2 phase cells [ (15.44% ± 0.53%) vs. (22.31% ± 0.20%), P < 0.05] and the rate of apoptosis [(3.45% ± 0.18%) vs. (5.45% ± 0.06%), P < 0.05] was significantly increased compared with the control group of HCC cells, and the differences were statistically significant. Conclusion: The expression of TUFT1 is highly expressed in hepatocellular carcinoma tissues. Furthermore, the expression of TUFT1 promotes HCC cell proliferation, inhibits the apoptosis, and is poor prognostic factor of hepatocellular carcinoma. 目的： 研究Tuftelin 1（TUFT1）的表达与肝细胞肝癌临床病理特征关系及其对肝癌细胞增殖凋亡的影响，探讨TUFT1与肝癌发生发展的关系。 方法： 应用免疫组织化学检测98例肝细胞肝癌及30例癌旁正常组织中TUFT1的表达，应用qRT-PCR的方法检测肝癌细胞系中TUFT1的表达，通过慢病毒载体介导敲减SMMC-7221细胞系中TUFT1的表达，采用细胞增殖检测、平板克隆形成试验、细胞凋亡实验、细胞周期检测检测下调TUFT1后肝癌细胞的增殖、凋亡能力及细胞周期的变化。统计学采用χ(2)检验及t检验。 结果： 98例肝细胞癌组织中TUFT1阳性表达65例（66.33%），30例癌旁正常组织中阳性表达6例（16.67%），差异有统计学意义（χ(2) = 19.956，P < 0.05）。肝癌组织中TUFT1表达与肿瘤大小、肿瘤分期及复发转移有关（χ(2)值分别为6.214、8.066、14.400，P值均< 0.05）。慢病毒载体介导敲减SMMC-7221细胞中TUFT1的表达后，与肝癌细胞SMMC-7221对照组比较，SMMC-7221细胞增殖率减少[（18.62%±0.15%）对比（67.91%±0.62%）]，克隆形成能力下降[（8.10%±0.80%）对比（50.80%±1.60%）]，G(1)期细胞明显减少[（36.71%±0.69%）对比（44.65%±0.73%）]，G(2)期细胞增加[（22.31%±0.20%）对比（15.44%±0.53%）]，细胞凋亡增加[（3.45%±0.18%）对比（5.45%±0.06%）]，P值均< 0.05，差异均有统计学意义。 结论： 肝癌组织中TUFT1高表达，TUFT1的表达促进肝癌细胞增殖、抑制细胞凋亡，是肝癌预后不良的因素。.

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