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  • Title: Effect of Ginsenoside Rg1 on the intervertebral disc degeneration rats and the degenerative pulposus cells and its mechanism.
    Author: Yu L, Hao Y, Peng C, Zhang P, Zhu J, Cai Y, Zhu G.
    Journal: Biomed Pharmacother; 2020 Mar; 123():109738. PubMed ID: 31951975.
    Abstract:
    OBJECTIVE: To explore the effect of ginsenoside Rg1 on intervertebral disc degeneration (IVDD) in vivo and in vitro and its mechanism. METHODS: 60 rats were underwent surgery to construct rat models of IVDD and divided in the sham group, model group and gradient G-Rg1 groups (10 mg/kg/d, 20 mg/kg/d and 40 mg/kg/d).The change of histology was observed by HE staining, the water content and the expression of β-catenin in IVD were detected. Rat nucleus pulposus cells(NPCs) were isolated from IVDD rats and divided in D-NPCs group, and gradient G-Rg1 groups(20 μg/ml, 50 μg/ml and 100 μg/ml).The cell proliferation activity, cell apoptosis rate,the expression of proteins related to ECM and Wnt/β-catenin were detected respectively, Finally the agonist of Wnt/β-catenin pathway LiCl was used for reversed experiments. RESULTS: In vivo, G-Rg1 treatment could improve the structural disorganization, low water content, NPCs number and aggrecan and collagenⅡ expression in IVD and down-regulate the expression of β-catenin. In vitro NPCs, G-Rg1 treatment could improve the low cell proliferation, high apoptosis rate and low expression of aggrecan and collagenⅡ in degenerative NPCs in a dose-dependent manner.G-Rg1 treatment could down-regulate the expression of proteins related to β-catenin signal and LiCl could reverse the increase of cell proliferation and ECM synthesis, decrease of apoptosis of degenerative NPCs induced by G-Rg1. CONCLUSION: G-Rg1 could promote ECM synthesis of degenerative NPCs and inhibiting its apoptosis, improve the IVDD via inhibiting the Wnt/β-catenin pathway.
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