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  • Title: White pepper-derived ratiometric carbon dots for highly selective detection and imaging of coenzyme A.
    Author: Long R, Guo Y, Xie L, Shi S, Xu J, Tong C, Lin Q, Li T.
    Journal: Food Chem; 2020 Jun 15; 315():126171. PubMed ID: 31991253.
    Abstract:
    A new-style white pepper derived dual-emission carbon dots (CDs) with a quantum yield of 10.4% was designed and facile constructed with one-pot solvothermal method. The green emission (520 nm) had an efficient and special "turn-on" fluorescence sensing of coenzyme A (CoA) with the aid of Cu2+, while red emission (668 nm) barely changed and worked as reference. In the concentration range (0-150 µM), relative fluorescence intensity ratios (F520/F668) showed excellent linear correlation with concentrations of CoA, and detection limit was as low as 8.75 nm. Moreover, the strategy has been successfully applied for label-free detection of CoA in real pig liver samples with good recoveries (93.3-108.0%). Notably, the synthesized CDs had durable fluorescence, low cytotoxicity, and good biocompatibility for cellular imaging, which demonstrated wide and promising applicability for biosensing and bioimaging in the future.
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