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  • Title: CRISPR/Cas9 Guide RNA Design Rules for Predicting Activity.
    Author: Hiranniramol K, Chen Y, Wang X.
    Journal: Methods Mol Biol; 2020; 2115():351-364. PubMed ID: 32006410.
    Abstract:
    A critical stage in performing gene editing experiments using the CRISPR/Cas9 system is the design of guide RNA (gRNA). In this chapter, we conduct a review of the current gRNA design rules for maximizing on-target Cas9 activity while minimizing off-target activity. In addition, we present some of the currently available computational tools for gRNA activity prediction and assay design.
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