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  • Title: Zinc oxide-gold nanocomposite as a proper platform for label-free DNA biosensor.
    Author: Hatami Z, Ragheb E, Jalali F, Tabrizi MA, Shamsipur M.
    Journal: Bioelectrochemistry; 2020 Jun; 133():107458. PubMed ID: 32006859.
    Abstract:
    In this study, a simple and cost-effective electrochemical DNA biosensor was developed for sensitive detection of mycobacterium tuberculosis (TB). Nanocomposite of zinc oxide (ZnO) and gold nanoparticles (AuNPs) was used as a platform for immobilizing thiolated TB DNA (probe DNA). ZnO was electrodeposited on a glassy carbon electrode by potentiostat electrolysis of Zn (NO3)2 solution at -1.0 V (vs. Ag/AgCl), then AuNPs were loaded as the second layer at -0.4 V from HAuCl4 solution. Thiolated probe DNA was then covalently attached to AuNPs. Anodic peak current of Fe (CN)63-/4- was followed in hybridization experiments and a linear calibration curve was obtained in concentration range of 2.5-250 pM and limit of detection (LOD) of 1.8 pM for target DNA. The label-free TB biosensor exhibited high selectivity, suitable stability, and reproducibility.
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