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  • Title: Optimal resolution of eye lens gamma-crystallins by cation-exchange high-performance liquid chromatography on SynChropak CM300.
    Author: Siezen RJ, Kaplan ED.
    Journal: J Chromatogr; 1988 Jul 01; 444():239-50. PubMed ID: 3204134.
    Abstract:
    Cation-exchange high-performance liquid chromatography on SynChropak CM300 in Tris-acetate buffers of pH 5-7, using sodium acetate gradients, produces an excellent separation of the various gamma-crystallin gene products and their post-synthetically modified forms from eye lens. With a single analysis of total lens extract, the gamma-crystallins can be resolved, quantified and collected for amino acid analysis. Experimental conditions are presented for optimal resolution of individual human, rat, bovine and dogfish shark gamma-crystallins. Applications presented include determinations of different synthesis of gamma-crystallins and chemical modification (oxidation by hydrogen peroxide) in situ.
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