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  • Title: [Proliferation of cultured cells with genetically induced resistance to ethidium bromide on synthetic nutrient media without serum].
    Author: Sal'nikov KV, Pashinin IuV, Ignatova TN.
    Journal: Tsitologiia; 1988 Aug; 30(8):991-8. PubMed ID: 3206546.
    Abstract:
    Lebr 625 and Lebr 350 cells, resistant to ethidium bromide in concentrations 25 and 50 mkg/ml, are able to grow continuously in serum- and protein-free media. Under the same conditions the parental L929 cells are not able to. Two cell lines (625 sf and 350 sf) were established capable of growing in serum- and protein free media. It is found that ethidium bromide is toxic for resistant cells grown the in serum-free medium. The addition of serum lowers the toxic action of ethidium bromide. A continuous growth of resistant cells in serum-free medium (under nonselective conditions) leads to a decreased level of resistance, which may nevertheless persist for a long period of cultivation (over 2.5 years).
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