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  • Title: [Early stage mechanical adaptability and osteogenic differentiation of mouse bone marrow derived mesenchymal stem cell under micro-vibration stimulation environment].
    Author: Wu J, Wu Y, Chen X, Zhi W.
    Journal: Sheng Wu Yi Xue Gong Cheng Xue Za Zhi; 2020 Feb 25; 37(1):96-104. PubMed ID: 32096382.
    Abstract:
    This study investigated the early mechanical adaptability and osteogenic differentiation of mouse bone marrow mesenchymal stem cells (M-BMSCs) under micro-vibration stimulation (MVS). M-BMSCs were stimulated by MVS in vitro, cell proliferation, alkaline phosphatase (ALP) activity assay, and cytoskeleton were measured, and cell apoptosis was observed by flow cytometry. Early osteoblast-associated genes, runt-related transcription factor 2 (Runx2), Collagen Ⅰ (Col-Ⅰ) and ALP, were observed by RT-PCR and the activation of extracellular regulated protein kinases 1/2 (ERK1/2) was determined by Western blotting. The results showed that MVS had no significant effect on the proliferation of M-BMSCs. The early apoptosis was induced by mechanical stimulation (for one day), but the apoptosis was decreased after cyclic stimulation for 3 days. At the same time, MVS significantly accelerated the expression of F-actin protein in cytoskeleton, the synthesis of ALP and the ERK1/2 pathway, also up-regulated the expressions of Runx2, Col-Ⅰ and ALP genes. This study indicates that MVS could regulate cellular activity, alter early adaptive structure and finally promote the early osteogenic differentiation of M-BMSCs. 本研究考察微振动刺激（MVS）对小鼠骨髓来源间充质干细胞（M-BMSCs）的早期力学适应性及成骨分化的调控作用。在体外给予 M-BMSCs 微振动刺激，检测细胞增殖及碱性磷酸酶（ALP）表达，荧光染色观察细胞凋亡与细胞骨架，流式检测细胞凋亡，通过 RT-PCR 检测早期成骨相关基因 runt 相关转录因子 2（Runx2）、Ⅰ型胶原（Col-Ⅰ）、ALP 表达，Western blotting 检测细胞外调节蛋白激酶 1/2（ERK1/2）磷酸水平。研究结果显示，微振动刺激诱导了细胞早期凋亡（受力 1 天后），但周期性受力 3 天后细胞的凋亡现象明显减少，而细胞的增殖活性并未受到明显影响；同时，微振动刺激促进了细胞骨架 F-actin 蛋白、ALP 合成和 ERK1/2 的磷酸化，并上调了 Runx2、Col-Ⅰ、ALP 的基因表达。研究结果表明微振动刺激能对 M-BMSCs 的细胞活性和结构产生早期适应性改变与调节，从而促进了细胞的早期成骨分化。.
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