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  • Title: First genomic characterization of a Belgian Enterovirus C104 using sequence-independent Nanopore sequencing.
    Author: Wollants E, Maes P, Merino M, Bloemen M, Van Ranst M, Vanmechelen B.
    Journal: Infect Genet Evol; 2020 Jul; 81():104267. PubMed ID: 32114255.
    Abstract:
    Because of the enormous variation in their genome sequence, genotyping enteroviruses by standard methods can prove to be quite challenging. Nanopore sequencing offers the potential to overcome the limitations of older techniques, but thus far, only amplicon-based strategies have been used to sequence complete enterovirus genomes. By combining a sequence-independent, single primer amplification (SISPA) for cDNA generation with next-generation sequencing using the Oxford Nanopore MinION, complete enterovirus genomes can be obtained in an easy-to-use, sequence-independent manner. To demonstrate its usability, we applied this technique to determine the complete genome sequence of an enterovirus C104 strain, representing the first documented occurrence of this uncommon enterovirus strain in Belgium.
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