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  • Title: Killing of Escherichia coli by a granulocyte fraction occurs without recognizable ultrastructural alterations in the bacterial envelope, as studied by freeze-fracture electron microscopy.
    Author: Van Houte AJ, Elsbach P, Verkleij A, Weiss J.
    Journal: Infect Immun; 1977 Feb; 15(2):556-9. PubMed ID: 321355.
    Abstract:
    Concentrations of a highly purified rabbit polymorphonuclear leukocyte fraction that rapidly caused irreversible loss of viability of Escherichia coli (S15) but reversible envelope alterations produced no recognizable morphological changes as studied by freeze-fracture electron microscopy. These findings support previous evidence that the killing of certain gram-negative microorganisms by granulocyte fractions occurs with minimal structural or functional disorganization of cytoplasmic and outer membranes.
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