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  • Title: Ultrasensitive competitive detection of patulin toxin by using strand displacement amplification and DNA G-quadruplex with aggregation-induced emission.
    Author: Zhang M, Wang Y, Sun X, Bai J, Peng Y, Ning B, Gao Z, Liu B.
    Journal: Anal Chim Acta; 2020 Apr 15; 1106():161-167. PubMed ID: 32145844.
    Abstract:
    A novel sensitive assay was established by using strand displacement amplification (SDA) and DNA G-quadruplex with aggregation-induced emission (AIE) for the detection of patulin (PAT) toxin. The complementary DNA (cDNA) of the aptamer and PAT competed for binding to aptamer-modified magnetic beads. The cDNA was obtained by magnetic separation and used as a primer in SDA to produce a large amount of G-base single-stranded DNA (ssDNA). They can form the G-quadruplex to be combined with the AIE of TTAPE dye, which features a special combination of G-quadruplex that amplify the fluorescent signals. This work can reach a lower detection limit of 0.042 pg mL-1 with a wide linear range of 0.001-100 ng mL-1 for PAT detection than other methods. The results also showed good recoveries of 97.8%-104% and 101.7%-105.3% in spiked apple and grape juices, respectively. The assay used for the detection of PAT exhibits high sensitivity and good specificity. It also provides a stable and reliable platform for detecting other small-molecule toxins.
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