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  • Title: Characterization of [3H]GABA release from striatal slices: evidence for a calcium-independent process via the GABA uptake system.
    Author: Bernath S, Zigmond MJ.
    Journal: Neuroscience; 1988 Nov; 27(2):563-70. PubMed ID: 3217004.
    Abstract:
    GABA can be released by depolarization even in the absence of external Ca2+. To investigate the underlying mechanism of this phenomenon, GABA release was studied using slices prepared from rat striatum. Slices were preincubated with [3H]GABA in the presence of beta-alanine and superfused with Krebs buffer. Total tritium efflux was measured as an index of GABA release. Electrical stimulation at 2 Hz for 3 min elevated resting tritium efflux approximately two-fold. Decreasing external Ca2+ to 0.1 mM increased basal tritium efflux and reduced electrically evoked overflow, while omitting Ca2+ entirely (and adding 1 mM EGTA) increased both basal efflux and evoked overflow. Tetrodotoxin (5 microM) abolished the evoked release of tritium but did not affect the resting outflow in either normal or Ca2+-deficient conditions. In the presence of normal Ca2+, nipecotic acid (0.1-1 mM), an inhibitor of GABA transport into neurons as well as glia, enhanced both spontaneous efflux and evoked overflow of tritium. Nipecotic acid also increased spontaneous release when external Ca2+ was reduced or removed; however, under these conditions electrically evoked overflow was reduced. These results suggest that the electrically evoked release of [3H]GABA from striatal slices is of neuronal origin, but can occur in part in the absence of external Ca2+. They further suggest that this Ca2+-independent release, which may co-exist with the Ca2+-dependent release, takes place via the same carrier system utilized for high-affinity GABA uptake.
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