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  • Title: Hybridization of DNA probes to A. marginale isolates from different sources and detection in Dermacentor andersoni ticks.
    Author: Ambrosio RE, Visser ES, Koekhoven Y, Kocan KM.
    Journal: Onderstepoort J Vet Res; 1988 Dec; 55(4):227-9. PubMed ID: 3217095.
    Abstract:
    DNA from the Washington, South-Idaho, Virginia and Florida isolates of Anaplasma marginale was hybridized to probes specific for Anaplasma centrale and A. marginale. The A. centrale probes AC-2 and AC-4 hybridized to identical bands on all of these isolates. The hybridization patterns suggests that the Virginia, Florida and the South African isolates are similar. A number of bands were obtained with the Washington isolate which differed from those obtained with the other isolates. Probe AC-2 could be developed to identify relatedness among Anaplasma isolates. Probe AC-2 detected A. marginale DNA in midgut material from infected Dermacentor andersoni ticks. No hybridization was obtained with DNA from salivary gland tissues from these infected ticks.
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