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  • Title: Towards high-efficient online specific discrimination of zearalenone by using gold nanoparticles@aptamer-based affinity monolithic column.
    Author: Xu J, Chi J, Lin C, Lin X, Xie Z.
    Journal: J Chromatogr A; 2020 Jun 07; 1620():461026. PubMed ID: 32178860.
    Abstract:
    Sensitive and specific analysis of zearalenone (ZEN) mycotoxin in cereals for ensuring food safety is critical and remains challenging. Herein, a new gold nanoparticles @aptamer-functionalized hybrid affinity monolithic column was proposed and employed for online specific recognition of ZEN by HPLC. Characterization on the morphology, Brunauer-Emmett-Teller (BET) surface area mechanical stability and specific performance of the obtained affinity monolith were investigated. A super-high aptamer coverage density could reach 3636 pmol/μL, which is preferable to gain an effective analysis of ZEN with high specificity and a low interference of co-existed substances including typical α-Zearalenol (α-ZOL) and Aflatoxin B1 (AFB1). The sensitive recognition of trace ZEN was obtained with the limit of detection (LOD) as low as 0.05 ng/mL. Applied to real cereal samples, satisfactory recoveries were obtained in the range of 91.6 ± 1.4%-97.8 ± 2.6% (n = 3) in corn, 93.8 ± 3.1%-95.0 ± 3.6% (n = 3) in wheat, and 90.9 ± 4.7%-94.7 ± 3.8% (n = 3) in rice, respectively. The results on quantitative analysis were similar to that of LC-MS and better than that obtained by using immunoaffinity column (IAC) or molecularly imprinted polymer (MIP). This protocol provided an efficient access to high-efficient online specific recognition of ZEN in cereals by using such an aptamer-affinity capillary monolithic column.
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