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  • Title: [Synthesis of tritium-labelled prostaglandin E1 and its binding to human platelets].
    Author: Shevchenko VP, Nagaev IIu, Vrzheshch PV, Ershov DE, Zaĭtsev SV.
    Journal: Bioorg Khim; 1988 Aug; 14(8):1075-85. PubMed ID: 3219124.
    Abstract:
    A method has been developed that makes it possible to obtain [5,6-3H2]PGE1 with a yield of 35% and a molar radioactivity of 1.7-1.8 TBq/mmol. The binding of [5,6-3H2]PGE1 to native platelets proved to be specific, saturating and reversible. It is characterized by low values (approximately 10(-9) M) of dissociation constants for high-affinity sites, correlates with the inhibition of ADP-induced aggregation of platelets and can be considered as receptor binding. Specific binding of 10 +/- 2 molecules of PGE1 with one platelet was found to cause 50% inhibition of the ADP-induced aggregation.
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