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  • Title: Determination of parameters for enzyme therapy using L-asparaginase entrapped in canine erythrocytes.
    Author: Naqi A, DeLoach JR, Andrews K, Satterfield W, Keeling M.
    Journal: Biotechnol Appl Biochem; 1988 Aug; 10(4):365-72. PubMed ID: 3219195.
    Abstract:
    The antitumor agent L-asparaginase was entrapped in canine erythrocytes by a single dialysis encapsulation (efficiency mean = 30%). Concentration of asparaginase in carrier cells was about 240 IU/ml, with an average of 62% cell recovery. Use of a double dialysis procedure increased the L-asparaginase concentration within carrier cells to 530 IU/ml, with an overall cell recovery of 53.9%. In vitro efflux experiments showed L-asparaginase-loaded canine carriers were stable at both 4 and 37 degrees C for an 18-h period. In vivo cell survival studies showed that carrier cells did circulate and that L-asparaginase had a half-life of 6.5 days. No evidence suggesting that the enzyme left the cell was found. Carrier cells prepared with [3H]inulin and [14C]sucrose were stored at 4 degrees C for 2 weeks and began to show signs of deterioration after 2 days.
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