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  • Title: [Tongjingling reduces ROS level and improves mitochondrial function in spermatogenic cells of varicocele rats with liver-qi stagnation].
    Author: Liu B, DU BX, Zheng JZ, Liu Q, Li PL, Ren GQ, Cui Y.
    Journal: Zhonghua Nan Ke Xue; 2018 Nov; 24(11):1021-1028. PubMed ID: 32212478.
    Abstract:
    OBJECTIVE: To explore the effects of the Chinese herbal compound Tongjingling (TJL) on the ROS level, mitochondrial membrane potential (MMP) and mitochondrial ultrastructure in spermatogenic cells in varicocele (VC) rats with liver-qi stagnation (LQS). METHODS: Totally, 72 male SPF Wistar rats were randomly divided into six groups of equal number, sham operation, VC+LQS model, low-, medium- and high-dose TJL, and L-carnitine (LC) control. The VC+LQS model was established by the Saypol method combined with tail-clamping. At 4 weeks after modeling, the rats in the low-, medium- and high-dose TJL groups were treated intragastrically with TJL at 0.6, 1.2 and 2.4 g/ml respectively, those in the sham operation and VC+LQS model groups with 0.9% saline, and those in the LC control group with LC oral liquid at 2.1 ml/kg, qd, for 8 weeks. Then the ROS level and MMP in the spermatogenic cells were measured by flow cytometry and the mitochondrial ultrastructure observed under the transmission electron microscope. RESULTS: The ROS level in the spermatogenic cells was significantly higher in the VC+LQS model and low-dose TJL groups than in the sham operation group (［62.72 ± 9.90］% and ［52.25 ± 8.15］% vs ［36.53 ± 5.25］%, P < 0.01), but remarkably lower in the medium-dose TJL (［41.65 ± 4.89］%), high-dose TJL (［39.60 ± 5.19］%) and LC control (［38.33 ± 7.46］%) than in the VC+LQS model group (P < 0.01). MMP in the spermatogenic cells was significantly lower in the VC+LQS model and low-dose TJL than in the sham operation group (［34.80 ± 20.18］% and ［44.39 ± 19.60］% vs ［60.24 ± 22.25］%, P < 0.01 and P < 0.05), but markedly higher in the medium-dose TJL (［50.62 ± 14.83］%), high-dose TJL (［52.42 ± 7.55］%) and LC control (［50.75 ± 15.65］%) than in the VC+LQS model group (P < 0.05). The mitochondria in the spermatogenic cells of the VC+LQS model rats exhibited swelling, vacuolization, rupture or disappearance of the mitochondrial cristae, and breakdown of the internal structure. The pathological injury of the mitochondrial structure was improved in different degrees in the TJL and LC groups. CONCLUSIONS: TJL can reduce the ROS level, increase MMP and improve pathological injury of the mitochondrial ultrastructure in the spermatogenic cells of VC rats with LQS, which may be the underlying molecular mechanism of TJL improving fertility in infertile VC patients.
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