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  • Title: Five hundredfold overproduction of DNA ligase after induction of a hybrid lambda lysogen constructed in vitro.
    Author: Panasenko SM, Cameron JR, Davis RW, Lehman IR.
    Journal: Science; 1977 Apr 08; 196(4286):188-9. PubMed ID: 322281.
    Abstract:
    A lambda vector that contains the gene for Escherichia coli DNA ligase (lambdagt4-lop-11 lig+) has been modified to achieve overproduction of this enzyme. The third Eco RI site in the lambda chromosome has been altered by mutation, and the left-hand Eco RI fragment has been shortened. The new vector, lambdagt4-lop-11 lig+, forms a stable lysogen which, upon induction, produces a 100-fold increase in DNA ligase activity. Introduction of a phage mutation (S7) that prevents cell lysis results in an even greater increase (500-fold).
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