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  • Title: Two-Step Bacterial Artificial Chromosome (BAC) Engineering: Electroporation of Competent BAC Host Cells with the Recombinant Shuttle Vector.
    Author: Heintz N, Gong S.
    Journal: Cold Spring Harb Protoc; 2020 Apr 01; 2020(4):098079. PubMed ID: 32238593.
    Abstract:
    Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.
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