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  • Title: Synergy effect of specific electrons and surface plasmonic resonance enhanced visible-light photoelectrochemical sensing for sensitive analysis of the CaMV 35S promoter.
    Author: Li H, Zhu M, Wang S, Chen W, Liu Q, Qian J, Hao N, Wang K.
    Journal: J Mater Chem B; 2017 Dec 07; 5(45):8999-9005. PubMed ID: 32264127.
    Abstract:
    Interest for accessible and reliable methods for detection of genetically modified organisms (GMOs) has been tremendously growing for providing a proper food label to consumers. Herein, we present a label-free and sensitive photoelectrochemical (PEC) detection of a transgene from the CaMV 35S promoter (P35S, the most commonly inserted gene) by utilizing a Ag/TiO2/nitrogen-doped graphene nanoribbon (Ag/TiO2/N-GNR) ternary composite as a photoactive material. The as-prepared ternary composite was synthesized via a facile one-pot heat treatment method and exhibited a significantly enhanced PEC activity owing to the synergy effect of the surface plasmatic resonance (SPR) of Ag nanoparticles and the rapid electron transfer of N-GNRs. Thus, it can broaden the visible light response and facilitate the charge transfer. Via the enhancement of the PEC response, a label-free PEC sensor for P35S was constructed to monitor the changes in the photocurrent signals of the PEC electrode resulting from DNA hybridization. Under the optimal conditions, a wide linear range for P35S detection was obtained from 0.01 to 500 nM, and the limit of detection (LOD) of the proposed PEC sensor was significantly lowered to 0.003 nM. The present study provides a new approach for the design of a versatile PEC sensor for the detection of nucleic acids with a low abundance for diagnostics and food safety control.
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