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  • Title: Mind your assays: Misleading cytotoxicity with the WST-1 assay in the presence of manganese.
    Author: Scarcello E, Lambremont A, Vanbever R, Jacques PJ, Lison D.
    Journal: PLoS One; 2020; 15(4):e0231634. PubMed ID: 32298350.
    Abstract:
    The WST-1 assay is the most common test to assess the in vitro cytotoxicity of chemicals. Tetrazolium-based assays can, however, be affected by the interference of tested chemicals, including carbon nanotubes or Mg particles. Here, we report a new interference of Mn materials with the WST-1 assay. Endothelial cells exposed to Mn particles (Mn alone or Fe-Mn alloy from 50 to 1600 μg/ml) were severely damaged according to the WST-1 assay, but not the ATP content assay. Subsequent experiments revealed that Mn particles interfere with the reduction of the tetrazolium salt to formazan. Therefore, the WST-1 assay is not suitable to evaluate the in vitro cytotoxicity of Mn-containing materials, and luminescence-based assays such as CellTiter-Glo® appear more appropriate.
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